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Pharmcia（GE）葡聚糖凝膠G-75

  • 更新時間：  2023-07-24
  • 產(chǎn)品型號：  Pharmcia17-0050-01
  • 簡單描述
  • Pharmcia（GE)葡聚糖凝膠G-75
    葡聚糖凝膠G-75，交聯(lián)葡聚糖凝膠G-75，交聯(lián)右旋糖酐凝膠G-75，Sephadex G-75
    分離:肽及球形蛋白質(zhì)：3000～80000；葡聚糖(線性分子)：1000～50000
詳細(xì)介紹

Pharmcia（GE)葡聚糖凝膠G-75

Sephadex G-75, 100 g

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
  • Highest exclusion limit, 80 000, in the Sephadex G-range
  • Classic gel filtration medium.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Pharmcia（GE)葡聚糖凝膠G-75

Sephadex G-75, 100 g

MatrixCross-linked dextran
Particle Size Wet90 µm-280 µm
Partical Size Dry40 µm-120 µm
pH Stability Working Range2-10
Flow Rate< Darcy´s Law1)
Pressure/Flow Specificationmin 90 cm/h, bed height 10 cm, column 5 cm i.d.
Fractionation [Mr] Globular Proteins3 x 103 – 8 x 104
Fractionation [Mr] Dextrans1 x 103-5 x 104
Exclusion Limit> 7 x 104
Storage Conditions4 to 30°C
AutoclavableIn wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

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